Translations in context of “herpesvirus canino” in Spanish-English from Reverso Context: Eurican Herpes contiene pequeñas cantidades de antígenos del. Canine herpesvirus infection. Actualización sobre herpesvirus canino 1 (CHV- 1) HERPESVIRUS CANINO 1: AGENTE ETIOLÓGICO Y ENFERMEDAD. Demonstration of canine herpesvirus-specific hemagglutination. Article (PDF HERPESVIRUS CANINO 1: AGENTE ETIOLÓGICO Y ENFERMEDAD. Article.
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Outbreaks of canid herpesvirus 1 disease in puppies in southern Brazil. Canid herpesvirus 1 CHV-1 is a widespread pathogen of dogs and produces infertility, abortions and severe systemic disease in young puppies.
Clinical data indicate the circulation of CHV-1 among Brazilian dogs yet definitive diagnosis has rarely been accomplished. Three events occurred in a kennel holding dogs of different breeds at reproductive age MarchOctober and April Puppies from three French or English Bulldog litters, aging 9 to 30 days were affected, presenting dyspnea, agonic breathing, pale mucous, abdominal pain and tension, evolving to death within about 24 hours.
At necropsy, the puppies presented necrohemorrhagic hepatitis, multifocal and moderate necrohemorrhagic nephritis and fibrinonecrotic interstitial pneumonia.
Phylogenetic analyses of gC sequences showed a segregation of the samples, even among three isolates from the same kennel. These findings support CHV-1 infection as the cause of acnino and death in these dog litters, reinforcing the need for correct etiologic diagnosis, prevention and immunization against CHV-1 in dogs from Southern Brazil.
Canid herpesvirus 1; CHV-1; diagnosis; dogs; glycoproteins and pathological findings. Canid herpesvirus 1 CHV-1 belongs to family Herpesviridaesubfamily Alphaherpesvirinae, genus Varicellovirus ICTV, and it is associated with a variety of clinical manifestations in dogs including neonatal deaths, respiratory, ocular, and reproductive disorders Evermann et al. Like other alphaherpesviruses, CHV-1 establishes latent infections in neural and non neural tissues after acute infection and can be reactivated in stressful and immunosuppressive situations Miyoshi et al.
The outcome and clinical manifestations of CHV-1 infection in dogs are associated with several risk factors, including age, breeding and kennel cough Ronsse et al. Adult dogs infected with CHV-1 usually develop a mild respiratory disease, frequently associated with Parainfluenza virus and Bordetella bronchiseptica infection Decaro et al.
The most important disorders associated with CHV-1 infection, however, generally occur in pregnant bitches and in young puppies. Systemic viremia in pregnant females may result in transplacentary CHV-1 transmission and fetal infection, leading to fetal deaths, abortion, mummification, infertility and the birth of weak puppies Hashimoto et al. The age of the puppies at the time of primary infection is determinant to the outcome of the disease and the clinical signs are usually more severe in neonates Decaro et al.
Hence, CHV-1 should be considered an important agent of disease and death in neonates and young dogs. Cases of disease clinically compatible with Cnino infection have been frequently reported by veterinarians in Brazil yet a definitive diagnosis is rarely reached. To date, only few reports were described in Herpesvjrus As no vaccine is commercially available in Brazil, CHV-1 infection of bitches and caanino puppies seem to occur endemically and, as such, have acquired relative importance for dog breeders and domestic dog owners in past years.
We hope our report contribute for the understanding of CHV-1 biology and, most importantly, herpesviris call attention to and help veterinarians in recognizing such cases. Three occurred from March to October fall to spring and the later occurred in April The main epidemiological and clinical findings are described below:. Two 9 to day-old puppies out of a litter of eight English Bulldogs presented severe dyspnea, pale mucous, abdominal pain and tension, with a fast evolution to death approx.
After birth, the bitch developed a severe mastitis that hampered breast-feeding. Four day-old litter of English Bulldog puppies developed respiratory agony and pulmonary edema that evolved to death in a few hours.
A day-old puppy of a litter of French Bulldogs presented nasal discharge, abdominal pustules and neurological signs, characterized by ataxia and incoordination.
The clinical course evolved to death in approximately 24 h. Two day-old puppies of a litter of six English Bulldogs developed abdominal pain and showed pale mucosae, with fast evolution to death 24 h. The bitch was seronegative to CHV-1 when introduced in the kennel, immediately before the pregnancy. Animals from all reported cases were submitted to necropsy and tissue samples kidney, lung and liver were collected for histopathology and virology diagnosis.
Thirteen serum samples collected from dogs and bitches of different breeds at reproductive age from the kennel cases 1, 3 and 4 were submitted to a standard virus-neutralization VN. The obtained sequences were analyzed by the Staden package Staden and aligned danino the BioEdit Sequence Alignment Editor Software suite, version 7.
To building the phylogenetic tree, based on sequences of gC gene, it was used the Neighbor-Joining method with bootstrap replicates implemented by MEGA5. At necropsy, all puppies had severe necrohemorrhagic disease, characterized by multifocal to aleatory petechia in liver and kidneys. In the lungs had severe edema and hemorrhage.
At histopathologic examination, there were necrohemorrhagic hepatitis, multifocal and moderate necrohemorrhagic nephritis Fig. No distemper virus-associated lesions were observed in any case differential diagnosis.
A Pulmonary lobes with severe edema and hemorrhage. B Aleatory foci of hemorrhage petechia over the renal cortex. C Multiple alveoli are necrotic and filled by fibrinous plaques and mild to moderate neutrophilic and histiocytic inflammatory infiltrate. D Severe necrosis of hepatocytes with Councilmann-like bodies and moderate hemorrhage. C, D Hematoxylin and eosin. The other samples remained negative for cpe after three passages.
Thirteen serum samples collected from dogs and bitches of different breeds at reproductive age were submitted to a standard virus-neutralization VN. Nine dogs presented antibodies to CHV-1 in titers ranging from 16 to These data demonstrate previous exposure to the virus since these dogs had no history of CHV-1 vaccination and, in addition, indicate that CHV-1 infection is probably endemic in the kennel.
As positive serology for CHV and for other alphaherpesviruses as well – indicate the condition of latent carrier, the cahino findings undercovered an important epidemiological situation in this kennel since seropositive animals may occasionally reactivate and transmit the virus. Thus, the virus is probably perpetuated in the kennel through periodic episodes of reactivation and transmission. The obtained gC sequences were then submitted to phylogenetic analysis using the Yerpesvirus method with bootstrap replicates implemented by MEGA5.
Phylogenetic tree based on the nucleotide sequences of glycoprotein C gene of Canid Herpesvirus 1. The tree was constructed using the Neigbohr-Joining method with 2, bootstrap replicates based on Tamura-3 parameters model and implemented by MEGA 5. Brazilian Gerpesvirus sample from other case Oliveira et al. herpesvrius
Translation of “herpesvirus canino” in English
The presumptive diagnosis of the four events was based on the clinical signs, high mortality and pathological findings. At necropsy, the macroscopic features herpesvieus typical of neonatal CHV-1 infection, characterized by a multisystemic necrohemorrhagic disease Decaro et al. In spite of the lack of published reports of CHV-1 infection and disease in Brazil – there are few exceptions such as Oliveira et al.
Virtually all suspected cases, however, remain without hsrpesvirus definitive etiological virology, serology, molecular cnino. In these particular cases, however, the support of a virology diagnostic laboratory allowed a definitive etiological diagnosis.
Positive virus isolation in primary canine cells, PCR and nucleotide sequencing confirmed the presumptive diagnosis. Subsequently, serology confirmed the circulation of the virus in the affected kennel.
In addition to the low body temperature, the lack of maternal immunity may worsen neonatal CHV-1 disease Evermann et al.
Maternal antibodies apparently protect puppies from fatal CHV-1 infection even though the animals develop subclinical infection Decaro et al.
In the present report, the affected puppies presented severe respiratory signs that evolved to death in a day or so.
Interestingly, a puppy from a French Bulldog litter Case 3 also presented neurological signs, which are rarely observed in puppies naturally infected with CHV For this reason, differential diagnosis for canine distemper virus CDVan agent involved in neurologic disease in dogs was performed Tipold et al.
Again, the pathological and virological findings were conclusive of CHV-1 infection and histopathological analyses dismissed CDV infection.
Sequence analysis of CHV-1 gC sequences including those from the reported cases and two obtained in GenBank revealed a high level of nucleotide identity. Two scenarios may be envisioned: The virus would remain latently in the dogs of the kennel and would be periodically reactivated and transmitted. The selective pressure upon the virus during the transmission-infection cycle would explain the few observed mutations.
Veterinary clinicians frequently describe clinical cases compatible with CHVinduced disease; but etiologic confirmation has been accomplished in rare occasions.
Outbreaks of canid herpesvirus 1 disease in puppies in southern Brazil
In this sense, this report contributes for the identification and awareness of a common cause of neonatal deaths in puppies in Southern Brazil, and indicates the herpesvirys for the adoption of control measures, including vaccination. Declaration of conflicting interests. Weiblen are CNPq research fellows. Clinical considerations of canine herpesvirus-infection.
Canine adenoviruses and herpesvirus. Canine reproductive, respiratory, and ocular diseases due to canine herpesvirus. Experimental transplacental transmission of canine herpesvirus in pregnant bitches during the 2nd trimester of gestation.
Clinical observations of canine herpesvirus. International Committee on Taxonomy of Viruses. Development of temperature regulation in the dog. Viral isolation and molecular identification of canine herpesvirus 1.
Naturally-occurring neonatal canine herpesvirus infection. Detection of canine herpesvirus DNA in the ganglionic neurons and the lymph node lymphocytes of latently infected dogs.
Herpes virus canino tipo 1 by Gabriela Franco Montessoro on Prezi
Clinic and cxnino findings in dogs naturally infected with canine herpesvirus. Risk factors and reproductive disorders associated with canine herpesvirus-1 CHV The Staden sequence analysis package. Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance and maximum parsimony methods. Neurological manifestations of canine-distemper virus-infection.
This is an open-access article distributed under the terms of the Creative Commons Attribution License. Services on Demand Journal. Introduction Canid herpesvirus 1 CHV-1 belongs to family Herpesviridaesubfamily Alphaherpesvirinae, genus Varicellovirus ICTV, and it is associated with a variety of clinical manifestations in dogs including neonatal deaths, respiratory, ocular, and reproductive disorders Evermann et al.
The main epidemiological and clinical findings are described below: Pathologic analyses Animals from all reported cases were submitted to necropsy and tissue samples kidney, lung and liver were collected for histopathology and virology diagnosis. Virus-neutralization Thirteen serum samples collected from dogs and bitches of herlesvirus breeds at reproductive age from the kennel cases 1, 3 and 4 were submitted to a standard virus-neutralization VN.
Results and Discussion At necropsy, all puppies had severe necrohemorrhagic disease, characterized by multifocal to aleatory petechia in liver herpesivrus kidneys. Conclusions Veterinary clinicians frequently describe clinical cases compatible with CHVinduced disease; but etiologic confirmation has been accomplished in rare occasions. October 31, ; Accepted: How to cite this article.