DOWNSTREAMING PROCESS PDF

This article throws light upon the five stages in downstream processing. The five stages are: (1) Solid-Liquid Separation (2) Release of Intracellular Products (3). Downstream Processing means recovery of desired products from its crude extract. As we all know that downstream processing is a procedure of separation as well as recovery of yielded products that are manufactured by.

Author: Tuzuru Kagashura
Country: Sweden
Language: English (Spanish)
Genre: Career
Published (Last): 19 August 2006
Pages: 400
PDF File Size: 17.84 Mb
ePub File Size: 15.54 Mb
ISBN: 556-7-56198-534-4
Downloads: 64047
Price: Free* [*Free Regsitration Required]
Uploader: Gokora

Downstream Processing :- An Overview and Process steps

Development of processes for isolation and purification of products is often a part of projects on fermentation, and in the recent years, to an increasing degree in projects targeting utilisation of marine biomass.

In addition to process development, products are also produced for characterization and for supply of smaller test quantities for procfss and collaboration partners.

For water-based separations, the department has equipment able to handle fermentation volumes up to l, and corresponding quantities of biomass. The equipment includes centrifuges, cell homogenizer, membrane filtration plants, and chromatography equipment.

  ELGAR O SALUTARIS HOSTIA PDF

Processes applying organic solvents can only be carried out in laboratory scale. By clicking the ” Agree “-button you are agreeing to our use of cookies. Find out more in our privacy policy in Norwegian only. eownstreaming

Stages in Downstream Processing: 5 Stages

Downstream processing — Product isolation, purification and characterization. Department of Biotechnology and Nanomedicine develops processes for fractionation and purification of products produced by donwstreaming, and from biomass.

The department has experience and infrastructure for purification of small, bioactive molecules, downstgeaming well as macromolecules such as proteins and virus. Small, bioactive moleculessuch as new compounds discovered by bioprospecting, are purified for structure elucidation and characterization of bioactivity.

These molecules often have low solubility in water, and methods involving extraction with organic solvents are applied. Preparative LC-MS is used prlcess purification, while other mass-spectrometric methods are applied for identification and structure elucidation.

For characterization of bioactivity a wide range of high-throughput bioassays have been established, based on microorganisms and higher cells.

Proteinsin particular enzymes, are purified by methods such as membrane filtration, adsorption and chromatography, as well as high-pressure homogenization for intracellular products. Similar methods are used for smaller, water soluble molecules, such as peptides and amino acids.

  CHARTE CANADIENNE DES DROITS ET LIBERTES PDF

Large-scale purification processes are developed for fermentation products and protein from biomass. Proteins are also purified for characterization, for instance enzymes with new, or modified, activities developed by enzyme evolution and engineering. The department has experience and a large toolbox for characterization of polysaccharides, with particular experience in alginate and alginate oligomers.

The department collaborates closely with NTNU http: Virus and nanoparticles are purified by field flow fractionation For water-based separations, the department has equipment able to handle fermentation volumes up to l, and corresponding quantities of biomass.

This site uses cookies By clicking the ” Agree “-button you are agreeing to our use of cookies.