Allicin terdapat pada enzim alliin dan allinase. Allicin tidak terdapat pada bawang segar melainkan dari bawang yang diiris tipis atau diuleg. Pada bawang putih. oil, for instance with steam distillation the allicin will decompose whereas extraction at (TLC) dari minyak bawang putih pada plat Si/ika Gel dengan eluen The effects of fresh garlic extract (FGE) against planktonic Candida spp. have been demonstrated in vitro and have been attributed to the action of allicin (3).
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SW Rochester, MN The effects of fresh bawnag extract FGE against planktonic Candida spp. Biofilm-associated, or sessile, Candida albicans organisms demonstrate increased resistance to traditional antifungal agents that have activity against their planktonic counterparts 5. Therefore, we investigated the activity of FGE against both planktonic and sessile forms of C.
FGE was prepared as previously described by Lemar and colleagues 7. One-third of the disks were placed in 0, 0. The other disks were incubated in yeast nitrogen base for 48 h to form mature biofilms.
All biofilms were evaluated with XTT [2,3-bis 2-methoxynitrosulfophenyl -2H-tetrazoliumcarboxanilide] reduction assay, a semiquantitative, colorimetric assay of metabolic activity 2. All experiments were performed in triplicate on three different days. MICs for planktonic yeast were 0. XTT assay results for adherent and biofilm-associated C. These data demonstrate activity of FGE against C.
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The in vitro activity decreases as the biofilm phenotype develops, as noted previously with traditional antifungal drugs 1. Although only one strain of C. These results appear promising and merit further investigation for determination of the antifungal activity of FGE against C. National Center for Biotechnology InformationU.
Journal List Antimicrob Agents Chemother v.
ShufordJames M. Author information Copyright and License information Disclaimer. This article has been cited by other articles in PMC.
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Open in a separate window. Acknowledgments We thank J. Douglas for use of the GDH isolate. Biofilm formation by the fungal pathogen Candida albicans: Antifungal resistance of candidal biofilms formed on denture acrylic in vitro.
Studies on the anticandidal mode of action of Allium aloicin garlic.